Signal transduction; Metabolic pathway; Endocrinology; Gastroenterology; Hormone metabolism
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Organism Species
Rattus norvegicus (Rat)
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Ghrelin (GHRL) . No significant cross-reactivity or interference between Mini Samples Ghrelin (GHRL) and analogues was observed.
Sensitivity
The minimum detectable dose of this kit is typically less than 51.5pg/mL
Detection Range
123.5-10,000pg/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Mini Samples Ghrelin (GHRL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Mini Samples Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Ghrelin (GHRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Ghrelin (GHRL) and unlabeled Mini Samples Ghrelin (GHRL) (Standards or samples) with the pre-coated antibody specific to Mini Samples Ghrelin (GHRL) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Ghrelin (GHRL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Ghrelin (GHRL) in the sample.
Assay Performance Time
3h
Method
Competitive Inhibition
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
