Enzyme-linked immunosorbent assay for Antigen Detection.
Organism Species
Rattus norvegicus (Rat)
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Atrial Natriuretic Peptide (ANP) . No significant cross-reactivity or interference between Mini Samples Atrial Natriuretic Peptide (ANP) and analogues was observed.
Sensitivity
The minimum detectable dose of this kit is typically less than 4.23pg/mL
Detection Range
12.35-1,000pg/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Mini Samples Atrial Natriuretic Peptide (ANP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Mini Samples Atrial Natriuretic Peptide (ANP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Atrial Natriuretic Peptide (ANP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Atrial Natriuretic Peptide (ANP) and unlabeled Mini Samples Atrial Natriuretic Peptide (ANP) (Standards or samples) with the pre-coated antibody specific to Mini Samples Atrial Natriuretic Peptide (ANP) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Atrial Natriuretic Peptide (ANP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Atrial Natriuretic Peptide (ANP) in the sample.
Assay Performance Time
3h
Method
Competitive Inhibition
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
