Torvigen TRV-047570-01 is a low sample volume sandwich ELISA kit listed for antigen detection of Matrix Metalloproteinase 2 (MMP2) in mouse research samples.
Product context
Catalog number: TRV-047570-01
Target: Matrix Metalloproteinase 2 (MMP2)
Organism species: Mus musculus (Mouse)
UniProt: P33434
Method: Double-antibody sandwich ELISA
Listed application: Enzyme-linked immunosorbent assay for antigen detection
Sample types: Serum, plasma, tissue homogenates, and other biological fluids
Assay performance time: 3 hours
Selection note: The visible product page lists a detection range of 0.156–10 ng/mL and a typical minimum detectable dose below 0.058 ng/mL. Confirm lot-specific values in the supplied datasheet before experimental use.
Search and entity terms
This product may be relevant to researchers searching for mouse MMP2 ELISA kit, Matrix Metalloproteinase 2 assay, gelatinase A ELISA, 72 kDa type IV collagenase assay, CLG4A, MMP-II, MONA, TBE-1, PEX, and low sample volume MMP2 antigen detection.
MMP2Mmp2Matrix Metalloproteinase 2Gelatinase A72 kDa Type IV CollagenaseMouse ELISATRV-047570-01
How researchers use this product
Laboratory teams may use this kit to measure mouse MMP2 antigen levels in compatible biological sample matrices during studies involving extracellular matrix remodeling, enzyme biology, tumor microenvironment research, infection immunity, or related biomarker workflows.
Supports low-volume ELISA workflows using 25 µL standards or samples per well as listed on the product page.
Uses a sandwich immunoassay format with HRP-based colorimetric detection and absorbance reading at 450 nm ± 10 nm.
Designed for comparative quantification against a standard curve within the listed assay range.
Selection notes for laboratory teams
Match the kit species to the intended study model: this product page lists Mus musculus (Mouse).
Review the listed sample types before ordering: serum, plasma, tissue homogenates, and other biological fluids are listed.
Confirm that the expected MMP2 concentration in your samples fits the listed 0.156–10 ng/mL detection range.
Confirm handling, storage, and disposal requirements against the batch-specific datasheet or SDS.
What is the Low Sample Volume ELISA Kit for Matrix Metalloproteinase 2 (MMP2) used for?
It is listed for enzyme-linked immunosorbent assay antigen detection of mouse Matrix Metalloproteinase 2 (MMP2) in research samples.
What is the target of TRV-047570-01?
The target is Matrix Metalloproteinase 2 (MMP2), also known as gelatinase A or 72 kDa type IV collagenase.
What applications are listed for this MMP2 ELISA kit?
The product page lists enzyme-linked immunosorbent assay for antigen detection using a double-antibody sandwich method.
What species is this MMP2 ELISA kit designed for?
The visible product specifications list Mus musculus (Mouse) as the organism species.
What sample types are listed for this kit?
The listed sample types are serum, plasma, tissue homogenates, and other biological fluids.
Is this product for research use only?
Yes. This product is for laboratory research use only and is not for human, veterinary, diagnostic, therapeutic, food, drug, cosmetic, or household use.
Research-use disclaimer: For laboratory research use only. Not for human, veterinary, diagnostic, therapeutic, food, drug, cosmetic, or household use.
Enzyme-linked immunosorbent assay for Antigen Detection.
Organism Species
Mus musculus (Mouse)
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Matrix Metalloproteinase 2 (MMP2) . No significant cross-reactivity or interference between Mini Samples Matrix Metalloproteinase 2 (MMP2) and analogues was observed.
Sensitivity
The minimum detectable dose of this kit is typically less than 0.058ng/mL
Detection Range
0.156-10ng/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Mini Samples Matrix Metalloproteinase 2 (MMP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Mini Samples Matrix Metalloproteinase 2 (MMP2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Matrix Metalloproteinase 2 (MMP2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Matrix Metalloproteinase 2 (MMP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Matrix Metalloproteinase 2 (MMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Matrix Metalloproteinase 2 (MMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
1. Prepare all reagents, samples and standards; 2. Add 25µ L standard or sample to each well. Incubate 1 hour at 37° C; 3. Aspirate and add 25µ L prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 25µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 25µ L Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 20µ L Stop Solution. Read at 450nm immediately.
Assay Performance Time
3h
Method
Double-antibody Sandwich
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
