ELISA Kit for Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) is a premium-quality life science research product supplied by Torvigen for laboratory and scientific applications.
Enzyme-linked immunosorbent assay for Antibody Detection.
Organism Species
Rattus norvegicus (Rat)
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) . No significant cross-reactivity or interference between Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) and analogues was observed.
Sensitivity
The minimum detectable dose of this kit is typically less than 1.09ng/mL
Detection Range
3.12-200ng/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Principle
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
