ELISA Kit for Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) is a premium-quality life science research product supplied by Torvigen for laboratory and scientific applications.
Enzyme-linked immunosorbent assay for Antibody Detection.
Organism Species
Mus musculus (Mouse)
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) . No significant cross-reactivity or interference between Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) and analogues was observed.
Sensitivity
The minimum detectable dose of this kit is typically less than 1.19ng/mL
Detection Range
3.12-200ng/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) and unlabeled Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) (Standards or samples) with the pre-coated antibody specific to Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Anti-Anti-Mullerian Hormone Antibody (Anti-AMH) in the sample.
Assay Protocol
1. Prepare all reagents, samples and standards; 2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 5 times; 5. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C; 6. Add 50µ L Stop Solution. Read at 450nm immediately.
