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CLIA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)
TRV-054996-01
CLIA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)
SKU:TRV-054996-01
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CLIA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) is a premium-quality life science research product supplied by Torvigen for laboratory and scientific applications.
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Product specifications
| Alternative Name | NT-Pro-BNP; ; N-BNP |
| Name Synonym | N-Terminal Pro-Brain Natriuretic Peptide |
| Uniprot | P07634 |
| Field of Research | Endocrinology; Cardiovascular biology |
| Applications | Chemiluminescent immunoassay for Antigen Detection. |
| Organism Species | Sus scrofa; Porcine (Pig) |
| Format | 48T, 96T, 96Tx5, 96Tx10, 96Tx100 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) . No significant cross-reactivity or interference between N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and analogues was observed. |
| Sensitivity | The minimum detectable dose of this kit is typically less than 5.5pg/mL |
| Detection Range | 13.7-10,000pg/mL |
| Precision | Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| Assay Principle | The microplate provided in this kit has been pre-coated with an antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) level in the sample or standard. |
| Assay Performance Time | 2h, 40min |
| Method | Double-antibody Sandwich |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids |