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CLIA Kit for Left/Right Determination Factor 1 (LEFTY1)

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TRV-046617-01

$322.16
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Product specifications

Alternative Name LEFTY-B; LEFTYB; LEFT-B; LEFTB; Left Right Determination Factor B
Name Synonym Left/Right Determination Factor 1
Uniprot O75610
Field of Research Neuro science
Applications Chemiluminescent immunoassay for Antigen Detection.
Organism Species Homo sapiens (Human)
Format 48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity This assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 1 (LEFTY1) . No significant cross-reactivity or interference between Left/Right Determination Factor 1 (LEFTY1) and analogues was observed.
Sensitivity The minimum detectable dose of this kit is typically less than 0.25pg/mL
Detection Range 0.69-500pg/mL
Precision Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Principle The microplate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Left/Right Determination Factor 1 (LEFTY1) level in the sample or standard.
Assay Protocol 1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.
Assay Performance Time 2h, 40min
Method Double-antibody Sandwich
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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