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CLIA Kit for Heat Shock 70kDa Protein 5 (HSPA5)

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TRV-048058-01

$330.26
$330.26
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CLIA Kit for Heat Shock 70kDa Protein 5 (HSPA5) is a premium-quality life science research product supplied by Torvigen for laboratory and scientific applications.

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Product specifications

Alternative Name MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78
Name Synonym Heat Shock 70kDa Protein 5
Uniprot P20029
Field of Research Signal transduction; Tumor immunity; Infection immunity
Applications Chemiluminescent immunoassay for Antigen Detection.
Organism Species Mus musculus (Mouse)
Format 48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity This assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 5 (HSPA5) . No significant cross-reactivity or interference between Heat Shock 70kDa Protein 5 (HSPA5) and analogues was observed.
Sensitivity The minimum detectable dose of this kit is typically less than 0.055ng/mL
Detection Range 0.156-10ng/mL
Precision Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Principle The microplate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 5 (HSPA5) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 5 (HSPA5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Heat Shock 70kDa Protein 5 (HSPA5) level in the sample or standard.
Assay Protocol 1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.
Assay Performance Time 2h, 40min
Method Double-antibody Sandwich
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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