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CLIA Kit for Follicle Stimulating Hormone (FSH)

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TRV-054103-01

$345.65
$345.65
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CLIA Kit for Follicle Stimulating Hormone (FSH) is a premium-quality life science research product supplied by Torvigen for laboratory and scientific applications.

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Product specifications

Name Synonym Follicle Stimulating Hormone
Field of Research Endocrinology; Reproductive science; Genetic science; Hormone metabolism
Applications Chemiluminescent immunoassay for Antigen Detection.
Organism Species Rattus norvegicus (Rat)
Format 48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity This assay has high sensitivity and excellent specificity for detection of Follicle Stimulating Hormone (FSH) . No significant cross-reactivity or interference between Follicle Stimulating Hormone (FSH) and analogues was observed.
Sensitivity The minimum detectable dose of this kit is typically less than 0.58ng/mL
Detection Range 1.56-400ng/mL
Precision Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Principle The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Follicle Stimulating Hormone (FSH) . A competitive inhibition reaction is launched between biotin labeled Follicle Stimulating Hormone (FSH) and unlabeled Follicle Stimulating Hormone (FSH) (Standards or samples) with the pre-coated antibody specific to Follicle Stimulating Hormone (FSH) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Follicle Stimulating Hormone (FSH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Follicle Stimulating Hormone (FSH) level in the sample or standard.
Assay Protocol 1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
        And then add 50µ L prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
7. Read RLU value immediately.
Assay Performance Time 2h
Method Competitive Inhibition
Sample Type Serum, plasma and other biological fluids

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