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CLIA Kit for Flotillin 2 (FLOT2)

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TRV-054994-01

$276.80
$276.80
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CLIA Kit for Flotillin 2 (FLOT2) is a premium-quality life science research product supplied by Torvigen for laboratory and scientific applications.

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Product specifications

Alternative Name ECS1; ESA; ESA1; M17S1; Epidermal Surface Antigen 1; Membrane Component, Chromosome 17, Surface Marker 1; 35kD Protein Identified By Monoclonal Antibody ECS-1
Name Synonym Flotillin 2
Uniprot Q14254
Field of Research Neuro science
Applications Chemiluminescent immunoassay for Antigen Detection.
Organism Species Homo sapiens (Human)
Format 48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity This assay has high sensitivity and excellent specificity for detection of Flotillin 2 (FLOT2) . No significant cross-reactivity or interference between Flotillin 2 (FLOT2) and analogues was observed.
Sensitivity The minimum detectable dose of this kit is typically less than 9.5pg/mL
Detection Range 27.4-20,000pg/mL
Precision Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Flotillin 2 (FLOT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Flotillin 2 (FLOT2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay Principle The microplate provided in this kit has been pre-coated with an antibody specific to Flotillin 2 (FLOT2) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Flotillin 2 (FLOT2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Flotillin 2 (FLOT2) level in the sample or standard.
Assay Performance Time 2h, 40min
Method Double-antibody Sandwich
Sample Type Tissue homogenates, cell lysates and other biological fluids

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